德國Emfret公司專注(zhu)心(xin)血管和血液系統(tong)研究(jiu)用抗體研(yan)發和生產(chan)，供應的(de)抗體包括清除(chu)血(xue)(xue)(xue)液中血(xue)(xue)(xue)小(xiao)板、流式細胞術鑒定分(fen)析(xi)小(xiao)鼠(shu)血(xue)(xue)(xue)小(xiao)板表面糖蛋(dan)白，emfret公司的(de)抗體不僅適用(yong)于(yu)流失(shi)分(fen)析(xi)、WB檢測(ce)，還可用(yong)于(yu)免疫(yi)組化/免疫(yi)熒光(guang)及免疫(yi)共沉淀檢測(ce)。

背景資料：

This antibody preparation contains a mixture of purified rat monoclonal antibodies directed against mouse GPIb (CD42b). Targeting of this receptor with divalent IgGs results in profound and irreversible Fc-independent platelet depletion in mice1,2 . Platelet depletion is frequently used to study the general role of platelets in (patho-) physiology3 .

保存條件：

The antibodies were purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography.

Stable for six months from date of shipment when stored at 4°C.

KEEP STERILE, the preparation contains no preservative. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.

應用：

This preparation is optimized to reduce the platelet count by >95% within 60 minutes after intravenous injection of 2 µg/g bodyweight. It is recommended to inject the antibodies in a volume of 100-200 µl (diluted with sterile PBS).

同型對照（抗體來源）： Rat IgG (Wistar)

緩沖液： 0.5 mg purified immunoglobulins in PBS, 0.2% (w/v) BSA, sterile filtered

濃度： 0.5 mg/ml

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實驗(yan)結果：