Virogen抗體貨號101-A文獻應用案例

品牌：Virogen

貨(huo)號：101-A

名(ming)稱(cheng)：ANTI-GLUTATHIONE MAB 100

論文題目(mu)：The effect of exercise and beta2-adrenergic stimulation on glutathionylation and function of the Na,K-ATPase in human skeletal muscle

期刊：Physiol Rep. 2015

摘要：運(yun)(yun)動(dong)(dong)(dong)時(shi)骨骼肌膜上(shang)的(de)(de)鉀和(he)(he)鈉位移(yi)可能(neng)(neng)(neng)導致(zhi)疲勞，部(bu)分受Na，K-ATP酶(mei)(mei)(mei)控(kong)制。因此，Na，K-ATP酶(mei)(mei)(mei)的(de)(de)調節(jie)對(dui)肌肉功能(neng)(neng)(neng)很重要。我們研究了(le)氧化(hua)(hua)(hua)(hua)(hua)應激（谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai)化(hua)(hua)(hua)(hua)(hua)）對(dui)Na，K-ATPase活(huo)(huo)(huo)性(xing)(xing)的(de)(de)影響。10 名男(nan)性(xing)(xing)受試者進行(xing)了(le) 3 次 4 分鐘的(de)(de)次極量運(yun)(yun)動(dong)(dong)(dong)，然后進行(xing)劇(ju)烈運(yun)(yun)動(dong)(dong)(dong)直至(zhi)筋(jin)疲力(li)盡(jin)，有和(he)(he)沒有特布他林的(de)(de) β2-腎(shen)上(shang)腺(xian)(xian)素能(neng)(neng)(neng)刺激。肌肉活(huo)(huo)(huo)檢(jian)(jian)是在休息時(shi)（對(dui)照樣本）和(he)(he)疲憊(bei)時(shi)從股外側分枝桿菌中獲(huo)得的(de)(de)。體外谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai)化(hua)(hua)(hua)(hua)(hua)以劑量依賴性(xing)(xing)方(fang)式降低(di)（P < 0.05）最大(da)Na，K-ATPase活(huo)(huo)(huo)性(xing)(xing)。Na，K-ATPase α亞(ya)基(ji)，通(tong)過免(mian)疫沉淀純(chun)化(hua)(hua)(hua)(hua)(hua)并通(tong)過谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai) （GSH） 抗(kang)體檢(jian)(jian)測，在對(dui)照樣品(pin)中具有基(ji)礎(chu)谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai)化(hua)(hua)(hua)(hua)(hua)，運(yun)(yun)動(dong)(dong)(dong)和(he)(he) β2-腎(shen)上(shang)腺(xian)(xian)素能(neng)(neng)(neng)刺激沒有進一(yi)(yi)步的(de)(de)谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai)化(hua)(hua)(hua)(hua)(hua)。使用抗(kang) GSH 抗(kang)體進行(xing)免(mian)疫沉淀，隨后使用 β1 抗(kang)體進行(xing)免(mian)疫檢(jian)(jian)測，結果顯示對(dui)照樣品(pin)中谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai)化(hua)(hua)(hua)(hua)(hua)率(lv)約(yue)為(wei) 20%，運(yun)(yun)動(dong)(dong)(dong)后谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai)化(hua)(hua)(hua)(hua)(hua) （至(zhi) 32%） 和(he)(he) β2-腎(shen)上(shang)腺(xian)(xian)素能(neng)(neng)(neng)刺激 （至(zhi) 38%，P < 0.05）。運(yun)(yun)動(dong)(dong)(dong)和(he)(he)β2-腎(shen)上(shang)腺(xian)(xian)素能(neng)(neng)(neng)刺激相結合，使β1谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai)化(hua)(hua)(hua)(hua)(hua)提高到45%（P < 0.05）。綜上(shang)所述，Na，K-ATP酶(mei)(mei)(mei)的(de)(de)α和(he)(he)β1亞(ya)基(ji)在對(dui)照樣品(pin)中均被谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai)化(hua)(hua)(hua)(hua)(hua)，這表明如(ru)果僅基(ji)于蛋白質密度，則Na，K-ATP酶(mei)(mei)(mei)的(de)(de)最大(da)活(huo)(huo)(huo)性(xing)(xing)被高估了(le)。β1 亞(ya)基(ji)通(tong)過運(yun)(yun)動(dong)(dong)(dong)和(he)(he) β2-腎(shen)上(shang)腺(xian)(xian)素能(neng)(neng)(neng)刺激進一(yi)(yi)步谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai)化(hua)(hua)(hua)(hua)(hua)。我們的(de)(de)數據表明，谷(gu)(gu)(gu)胱(guang)甘(gan)(gan)肽(tai)化(hua)(hua)(hua)(hua)(hua)有助于人骨骼肌中Na，K-ATP酶(mei)(mei)(mei)功能(neng)(neng)(neng)的(de)(de)復(fu)雜調節(jie)。Na，K-ATP酶(mei)(mei)(mei)的(de)(de)谷(gu)(gu)(gu)氨酰化(hua)(hua)(hua)(hua)(hua)可以解釋運(yun)(yun)動(dong)(dong)(dong)后最大(da)Na，K-ATPase活(huo)(huo)(huo)性(xing)(xing)的(de)(de)降低(di)，這可能(neng)(neng)(neng)與肌肉疲勞有關。