中文摘(zhai)要：

為了研(yan)究(jiu)卵(luan)(luan)(luan)(luan)巢(chao)(chao)巨(ju)噬細胞(bao)(bao)群體(ti)(ti)在(zai)排(pai)(pai)(pai)卵(luan)(luan)(luan)(luan)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)作用，我們研(yan)究(jiu)了使用脂(zhi)質(zhi)(zhi)體(ti)(ti)封裝的(de)(de)(de)(de)(de)(de)(de)(de)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)鹽消耗該群體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)影響。在(zai)排(pai)(pai)(pai)卵(luan)(luan)(luan)(luan)前 84 小時（第(di)(di)(di)(di) -3 天）或 36 小時（第(di)(di)(di)(di) -1 天）在(zai)促性(xing)(xing)(xing)腺激(ji)素引發(fa)的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)年小鼠的(de)(de)(de)(de)(de)(de)(de)(de)卵(luan)(luan)(luan)(luan)巢(chao)(chao)滑囊下注射(she)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)質(zhi)(zhi)體(ti)(ti)、鹽水(shui)脂(zhi)質(zhi)(zhi)體(ti)(ti)或單獨生理鹽水(shui)。排(pai)(pai)(pai)卵(luan)(luan)(luan)(luan)率是通過(guo)計算釋放的(de)(de)(de)(de)(de)(de)(de)(de)卵(luan)(luan)(luan)(luan)母細胞(bao)(bao)數量(liang)來確定的(de)(de)(de)(de)(de)(de)(de)(de)。排(pai)(pai)(pai)卵(luan)(luan)(luan)(luan)前后(hou)也立(li)即計算了graafian卵(luan)(luan)(luan)(luan)泡和(he)黃體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)數量(liang)。使用針對巨(ju)噬細胞(bao)(bao)抗(kang)原 macrosialin、主要組(zu)織相容性(xing)(xing)(xing)復合物 II 類 （Ia） 和(he) F4/80 的(de)(de)(de)(de)(de)(de)(de)(de)特異性(xing)(xing)(xing)單克隆(long)抗(kang)體(ti)(ti)進(jin)行免疫組(zu)化檢查(cha)排(pai)(pai)(pai)卵(luan)(luan)(luan)(luan)前卵(luan)(luan)(luan)(luan)巢(chao)(chao)的(de)(de)(de)(de)(de)(de)(de)(de) theca 和(he)基質(zhi)(zhi)內(nei)的(de)(de)(de)(de)(de)(de)(de)(de)巨(ju)噬細胞(bao)(bao)分(fen)布(bu)。在(zai)第(di)(di)(di)(di) -1 天注射(she)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)質(zhi)(zhi)體(ti)(ti)不影響排(pai)(pai)(pai)卵(luan)(luan)(luan)(luan)率，而在(zai)第(di)(di)(di)(di) -3 天注射(she)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)質(zhi)(zhi)體(ti)(ti)導致排(pai)(pai)(pai)卵(luan)(luan)(luan)(luan)率顯著降(jiang)低（平均排(pai)(pai)(pai)卵(luan)(luan)(luan)(luan)卵(luan)(luan)(luan)(luan)母細胞(bao)(bao)分(fen)別為 5. 25 +/- 0.6 來自(zi)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)質(zhi)(zhi)體(ti)(ti)處理的(de)(de)(de)(de)(de)(de)(de)(de)卵(luan)(luan)(luan)(luan)巢(chao)(chao)和(he) 9.13 +/- 0.9 來自(zi)鹽水(shui)處理的(de)(de)(de)(de)(de)(de)(de)(de)卵(luan)(luan)(luan)(luan)巢(chao)(chao)，P < 0.05）。排(pai)(pai)(pai)卵(luan)(luan)(luan)(luan)時 theca 中(zhong)存(cun)在(zai)的(de)(de)(de)(de)(de)(de)(de)(de)巨(ju)唾液(ye)酸(suan)(suan)(suan)陽(yang)性(xing)(xing)(xing)巨(ju)噬細胞(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)數量(liang)通過(guo)第(di)(di)(di)(di) -1 天的(de)(de)(de)(de)(de)(de)(de)(de)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)質(zhi)(zhi)體(ti)(ti)處理減少(shao)(shao)，第(di)(di)(di)(di) -3 天的(de)(de)(de)(de)(de)(de)(de)(de)治療減少(shao)(shao)了 theca 中(zhong)存(cun)在(zai)的(de)(de)(de)(de)(de)(de)(de)(de) Ia 陽(yang)性(xing)(xing)(xing)和(he)巨(ju)唾液(ye)素陽(yang)性(xing)(xing)(xing)巨(ju)噬細胞(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)數量(liang)。當通過(guo)陰道(dao)涂(tu)片(pian)檢查(cha)隨后(hou)的(de)(de)(de)(de)(de)(de)(de)(de)卵(luan)(luan)(luan)(luan)巢(chao)(chao)周期時，發(fa)現(xian)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)質(zhi)(zhi)體(ti)(ti)處理的(de)(de)(de)(de)(de)(de)(de)(de)動物的(de)(de)(de)(de)(de)(de)(de)(de)中(zhong)胚層-2/二元期延長（7.5 +/- 1.3天，鹽水(shui)脂(zhi)質(zhi)(zhi)體(ti)(ti)處理動物為3.4 +/- 0.4天，P < 0.05）。這些結果表(biao)明，鞘狀(zhuang)巨(ju)噬細胞(bao)(bao)可能參與卵(luan)(luan)(luan)(luan)泡生長和(he)破裂的(de)(de)(de)(de)(de)(de)(de)(de)調節(jie)，并且對發(fa)情周期的(de)(de)(de)(de)(de)(de)(de)(de)正常進(jin)展(zhan)很重要。

英文摘(zhai)要：

To investigate the role of the ovarian macrophage population in ovulation, we examined the effect of depleting this population using liposome-encapsulated clodronate. Clodronate liposomes, saline liposomes, or saline alone was injected under the ovarian bursa in gonadotropin-primed adult mice, either 84 h (Day -3) or 36 h (Day -1) before ovulation. Ovulation rates were determined by counting the number of oocytes released. The numbers of graafian follicles and corpora lutea were also counted immediately before and after ovulation. Macrophage distribution within the theca and stroma of preovulatory ovaries was examined by immunohistochemistry with specific monoclonal antibodies to the macrophage antigens macrosialin, major histocompatability complex class II (Ia), and F4/80. Injection of clodronate liposomes on Day -1 did not affect ovulation rates, whereas administration on Day -3 caused a significant reduction in ovulation rate (mean oocytes ovulated = 5. 25 +/- 0.6 from clodronate liposome-treated ovaries and 9.13 +/- 0.9 from saline-treated ovaries, respectively, P < 0.05). The numbers of macrosialin-positive macrophages present in the theca at ovulation were reduced by treatment with clodronate liposomes on Day -1, and treatment on Day -3 reduced the numbers of Ia-positive and macrosialin-positive macrophages present in the theca. When the subsequent ovarian cycles were examined by vaginal smearing, the metestrous-2/diestrous stage was found to be extended in clodronate liposome-treated animals (7.5 +/- 1.3 days vs. 3.4 +/- 0.4 days for saline liposome-treated animals, P < 0.05). These results suggest that thecal macrophages may be involved in the regulation of follicular growth and rupture, as well as being important for the normal progression of the estrous cycle.

產品詳情(qing)：

品(pin)牌(pai)：LIPOSOMA

貨號：CP-005-005
產地：荷蘭

品(pin)名：Clodronateliposomes氯(lv)膦(lin)酸二鈉脂質體巨噬細胞清除劑

實驗細節：

實驗結果：

論文題目：

ntrabursal injection of clodronate liposomes causes macrophage depletion and inhibits ovulation in the mouse ovary。

該文章(zhang)(zhang)比較(jiao)久(jiu)了，是較(jiao)早研究卵巢巨噬細胞的(de)文章(zhang)(zhang)，可以(yi)借鑒。因(yin)為研究很(hen)早，所以(yi)數據(ju)很(hen)基礎(chu)。無(wu)論如何(he)，我們可以(yi)訂購荷蘭liposoma品牌的(de)巨噬細胞清除劑Clodronateliposomes，具體訂購聯系靶點(dian)科技。這為研究清除卵巢巨噬細胞提供的(de)研究基礎(chu)。